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Product Code: QCP-008-10

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Specific particle surface chemistry enables a broad range of coating and binding applications. SPHERO functionalised polystyrene particles provide reactive groups on uniform microparticles for consistent and repeatable coating and binding. There are several ways to prepare particles with functionalised surfaces - Polymerization initiator selection - Functionalised monomer grafting. A variety of functional groups can be provided on the microparticle's exterior surface by selecting the appropriate polymerization initiator. For instance, if potassium persulphate is used as an initiator for polymerization the particle will have sulfate groups. Similarly, other functional groups can be introduced on the surface of the particles by using other functionalised initiators. Another method for providing surface functional groups is by grafting functionalised monomer after the polymerization process. This type of functionalised polystyrene particles are prepared by coating a thin layer of functionalised monomer onto the surface of plain particles. As a result, all of the functional groups are on the surface of the particles. The functional groups are attached to the surface of the particles by alkyl chains of two to eight carbons in length depending upon the type of functionalised monomer used. Below are the surface charge densities for 0.8 um carboxyl and amino particles: - SPHERO carboxyl polystyrene particles with a diameter of 0.8 um typically contain ~ 50 ueq/g of carboxyl groups on their surface. - SPHERO amino polystyrene particles with a diameter of 0.8 um typically contain ~ 15 to 20 ueq/g of amino groups on their surface. Nonetheless, the choice of particle size and type is dependent upon the intended application. For instance, particles with size of 0.4 to 2.0 um are suitable for latex agglutination assay, solid phase enzyme immunoassay or solid phase fluorescence immunoassay. Particles with size of 2.0 um or larger are preferred for flow cytometry applications.

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