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Lowicryl K11M kit (DG) Lowicryl K11M kit (DG)

This product can only be shipped by road.

Prices are current as of Sunday, 25/08/2019.
Prices are GST exclusive. Tax is added at checkout.

$1,379.00
$928.00
Availability: 2 In Stock
Product Code: C450

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Lowicryl resins are highly cross-linked acrylate-based embedding resins, designed for use over a wide range of embedding conditions. These resins provide low viscosity at low temperatures. The K4M kit is usable to -35 deg. C and is a polar (hydrophilic) medium, while the HM20 kit can be used down to -70 deg. C and is nonpolar (hydrophobic). Lowicryl's K11M and HM23 have properties similar to K4M and HM20, but can be used at temperatures 20 degrees lower than others: K11M at -60 deg. C, HM23 at least -80 deg. C. All of these resins are photopolymerised by long wavelength (360nm) ultraviolet light. Since the initiation of the polymerisation is largely independent of temperatures, blocks may be polymerised at the same temperatures used for infiltration. The resin may also be chemically polymerised at 60 deg. C.  

The hydrophilic properties of K4M and K11M provide two distinct advantages. During dehydration and infiltration the specimens may be kept in partially hydrated state since K4M and K11M may be polymerised with up to 5% (by weight) water in the block. Secondly, K4M and K11M are particularly useful for immunolabelling of sections using specific antisera, lectins, and colloidal gold particles (references 5-8) . The use of K4M or K11M results in a better structural preservation (references 9) , and improved preservation of antigenicity and a significantly lower background labelling (references 10-15) .

Lowicryl MonoStep Single Component Embedding Media
The same as our Lowicryl complete kits but this new formulation allows you one stop embedding without all of the cumbersome preparation and mixing. This pre-mixed, ready to use resin, saves you time and minimises chemical contact. MonoStep Lowicryl is ideal for use in immunohistochemistry and immunolabelling. Store at room temperature.

The resins should be prepared in brown glass containers or otherwise protected from direct light. All the components are miscible with each other. Avoid excessive stirring, which may result in the incorporation of oxygen into the resin, thereby interfering with the polymerisation. Gentle stirring with a glass rod for 3 to 5 minutes or mixing with a stream of dry nitrogen gas bubbled through resin is recommended.

The mixture below gives blocks with a medium hardness for Ultraviolet Polymerisation:

K4M HM20 K11M HM23**
Crosslinker A 2.70g Crosslinker D 2.98g Crosslinker 1.0g Crosslinker 1.1g
Monomer B 17.30g Monomer E 17.02g Monomer 19.0g Monomer 18.9g
Initiator C 0.10g Initiator C* 0.10g Initiator 0.1g Initiator 0.1g

*for polymerisation of K4M or HM20 between -50°C to 0°C. Above 0°C, the initiator can be replaced by the same amount of benzoin ethyl ether. Room temperature polymerised blocks can be ready for sectioning after a few hours.

A typical dehydration infiltration schedule for ethanol, which may be used for Lowicryl ® resin, is given as follows:

Ethanol, Vol % Temperature ( °C) Time
30% 0 30 min.
50% -20 60 min.
70% -35 (-50)* 60 min.
95% -35 (-50 to -80)* 60 min.
100% -35 (-50 to -80)* 60 min.
100% -35 (-50 to -80)* 60 min.
     
Ethanol, vol:vol Temperature °C Time
1:1 -35 (-50 to -80)* 60 min.
2.1 -35 (-50 to -80)* 60 min.
Pure resin -35 (-50 to -80)* Overnight or
4-16 hours

* suggested steps for work at lower temperature possible only with HM20 (-70°C), HM23 (-80°C) and K11M (-60°C)

At all temperatures below 0°C, care must be taken not to allow the residual water in the specimen to freeze during the dehydration step.

A variety of schemes can be developed for any other temperature or polar dehydrating agent, as long as solubility allows it, including freeze substitution methods.

The biological consequences of using still lower temperatures have to be explored. Nothing precise is known as yet about the completeness of dehydration at these two temperatures. Lack of success, due to a late low of water, e.g. in the pyramid of the block, should not automatically be interpreted as a "bad infiltration". The demonstrated persistence of lipids at lower temperatures seems not to cause problems of infiltration (references 19).

Polymerization

The resin is polymerised by direct long-wave length UV-irradiation 360nm 2x15 watt (Philips TLAD 15W/05 or equivalent) at -30°C to -40°C at a distance of 30-40 cm for 24 hours with EMS High Intensity Lamp (100 watt), Cat. #72414, keep distance greater than 85cm). Hardening can be done in filled to capacity gelatine or BEEM® capsules. Slow polymerization, particularly using diffuse radiation, produces superior blocks without severe shrinkage effects. Sectionable preparations can be produced in as little as 12 hours, through the sectioning quality improves when they are further hardened under UV light at room temperature for 2-3 days.

Sectioning and Staining

Lowicryl® easily yields silver to gray sections on diamond or glass knives. Optimal sectioning can be done easily with uranyl acetate and Reynold's lead citrate. Oxidizing heavy metal stains like OsO4 or KMnO4 produce inferior results due to the reaction with the resin.

K4M and K11M are hydrophilic resins. Therefore, as with other polar (water-miscible) resins, precautions should be taken to insure that the block face does not become wet during sectioning. Sections should be collected as soon as possible after they are cut, and staining incubation should be kept as brief as possible .

Precautions and Storage

The chemical, physical and toxicological properties of these products are not fully known. Avoid contacts with skin and eyes. Avoid inhalation of resin vapour. Use well-ventilated fume hood for mixing resins. Although not as toxic as epoxy resins, it has been shown that methacrylate resins can cause irritation to skin and eyes, and may cause sensitisation to some individuals. Vinyl or PVC gloves are recommended (EMS Cat. #71108). The use of disposable utensils and tools is recommended. Note: Kits should not be stored in the refrigerator.

In case of contact, promptly wash with plenty of soap and water. Flush eyes with plenty of water. Get medical attention immediately.

C450 contains: Monomer I, Crosslinker H, Initiator C
C440 contains: 3 x 225mL Monomer E, 1 x 130g Crosslinker D, 4.8g Initiator C

C430 Lowicryl K4M MSDS - ask
C435 Lowicryl K4M monostep (polar) MSDS - ask
C460 Lowicryl HM23 MSDS - ask

Lowicryl resins are highly cross-linked acrylate-based embedding resins, designed for use over a wide range of embedding conditions. These resins provide low viscosity at low temperatures. The K4M kit is usable to -35 deg. C and is a polar (hydrophilic) medium, while the HM20 kit can be used down to -70 deg. C and is nonpolar (hydrophobic). Lowicryl's K11M and HM23 have properties similar to K4M and HM20, but can be used at temperatures 20 degrees lower than others: K11M at -60 deg. C, HM23 at least -80 deg. C. All of these resins are photopolymerised by long wavelength (360nm) ultraviolet light. Since the initiation of the polymerisation is largely independent of temperatures, blocks may be polymerised at the same temperatures used for infiltration. The resin may also be chemically polymerised at 60 deg. C.  

The hydrophilic properties of K4M and K11M provide two distinct advantages. During dehydration and infiltration the specimens may be kept in partially hydrated state since K4M and K11M may be polymerised with up to 5% (by weight) water in the block. Secondly, K4M and K11M are particularly useful for immunolabelling of sections using specific antisera, lectins, and colloidal gold particles (references 5-8) . The use of K4M or K11M results in a better structural preservation (references 9) , and improved preservation of antigenicity and a significantly lower background labelling (references 10-15) .

Lowicryl MonoStep Single Component Embedding Media
The same as our Lowicryl complete kits but this new formulation allows you one stop embedding without all of the cumbersome preparation and mixing. This pre-mixed, ready to use resin, saves you time and minimises chemical contact. MonoStep Lowicryl is ideal for use in immunohistochemistry and immunolabelling. Store at room temperature.

The resins should be prepared in brown glass containers or otherwise protected from direct light. All the components are miscible with each other. Avoid excessive stirring, which may result in the incorporation of oxygen into the resin, thereby interfering with the polymerisation. Gentle stirring with a glass rod for 3 to 5 minutes or mixing with a stream of dry nitrogen gas bubbled through resin is recommended.

The mixture below gives blocks with a medium hardness for Ultraviolet Polymerisation:

K4M HM20 K11M HM23**
Crosslinker A 2.70g Crosslinker D 2.98g Crosslinker 1.0g Crosslinker 1.1g
Monomer B 17.30g Monomer E 17.02g Monomer 19.0g Monomer 18.9g
Initiator C 0.10g Initiator C* 0.10g Initiator 0.1g Initiator 0.1g

*for polymerisation of K4M or HM20 between -50°C to 0°C. Above 0°C, the initiator can be replaced by the same amount of benzoin ethyl ether. Room temperature polymerised blocks can be ready for sectioning after a few hours.

A typical dehydration infiltration schedule for ethanol, which may be used for Lowicryl ® resin, is given as follows:

Ethanol, Vol % Temperature ( °C) Time
30% 0 30 min.
50% -20 60 min.
70% -35 (-50)* 60 min.
95% -35 (-50 to -80)* 60 min.
100% -35 (-50 to -80)* 60 min.
100% -35 (-50 to -80)* 60 min.
     
Ethanol, vol:vol Temperature °C Time
1:1 -35 (-50 to -80)* 60 min.
2.1 -35 (-50 to -80)* 60 min.
Pure resin -35 (-50 to -80)* Overnight or
4-16 hours

* suggested steps for work at lower temperature possible only with HM20 (-70°C), HM23 (-80°C) and K11M (-60°C)

At all temperatures below 0°C, care must be taken not to allow the residual water in the specimen to freeze during the dehydration step.

A variety of schemes can be developed for any other temperature or polar dehydrating agent, as long as solubility allows it, including freeze substitution methods.

The biological consequences of using still lower temperatures have to be explored. Nothing precise is known as yet about the completeness of dehydration at these two temperatures. Lack of success, due to a late low of water, e.g. in the pyramid of the block, should not automatically be interpreted as a "bad infiltration". The demonstrated persistence of lipids at lower temperatures seems not to cause problems of infiltration (references 19).

Polymerization

The resin is polymerised by direct long-wave length UV-irradiation 360nm 2x15 watt (Philips TLAD 15W/05 or equivalent) at -30°C to -40°C at a distance of 30-40 cm for 24 hours with EMS High Intensity Lamp (100 watt), Cat. #72414, keep distance greater than 85cm). Hardening can be done in filled to capacity gelatine or BEEM® capsules. Slow polymerization, particularly using diffuse radiation, produces superior blocks without severe shrinkage effects. Sectionable preparations can be produced in as little as 12 hours, through the sectioning quality improves when they are further hardened under UV light at room temperature for 2-3 days.

Sectioning and Staining

Lowicryl® easily yields silver to gray sections on diamond or glass knives. Optimal sectioning can be done easily with uranyl acetate and Reynold's lead citrate. Oxidizing heavy metal stains like OsO4 or KMnO4 produce inferior results due to the reaction with the resin.

K4M and K11M are hydrophilic resins. Therefore, as with other polar (water-miscible) resins, precautions should be taken to insure that the block face does not become wet during sectioning. Sections should be collected as soon as possible after they are cut, and staining incubation should be kept as brief as possible .

Precautions and Storage

The chemical, physical and toxicological properties of these products are not fully known. Avoid contacts with skin and eyes. Avoid inhalation of resin vapour. Use well-ventilated fume hood for mixing resins. Although not as toxic as epoxy resins, it has been shown that methacrylate resins can cause irritation to skin and eyes, and may cause sensitisation to some individuals. Vinyl or PVC gloves are recommended (EMS Cat. #71108). The use of disposable utensils and tools is recommended. Note: Kits should not be stored in the refrigerator.

In case of contact, promptly wash with plenty of soap and water. Flush eyes with plenty of water. Get medical attention immediately.

C450 contains: Monomer I, Crosslinker H, Initiator C
C440 contains: 3 x 225mL Monomer E, 1 x 130g Crosslinker D, 4.8g Initiator C

C430 Lowicryl K4M MSDS - ask
C435 Lowicryl K4M monostep (polar) MSDS - ask
C460 Lowicryl HM23 MSDS - ask

Shipping

Orders with items in stock are packed within 24 hours. We ship with our best choice of courier, depending on goods ordered and shipping address provided.

Location

Standard delivery

Express delivery

Townsville/Local

Next day

N/A

Queensland

2-3 business days

N/A

NSW

4-10 business days

2-3 business days

SA/Tas/Vic/WA

6-14 business days

2-3 business days

Overseas

N/A

5-14 business days

*Note: Delivery times are as provided by our carriers and are estimates only. Delays may occur during peak periods, and due to factors outside the carriers' control.

Standard shipping is $15 in Australia (free on orders over $200). Add $5 to ship Express (under 2kg only)

Pickup

Order online and collect from our warehouse in Townsville.

Location

Freight Charge

Ready for collection

11 Carlton Street, Kirwan

$0

Once invoice has been emailed
(Less than 1 business day)



Shipping

Orders with items in stock are packed within 24 hours. We ship with our best choice of courier, depending on goods ordered and shipping address provided.

Location

Standard delivery

Express delivery

Townsville/Local

Next day

N/A

Queensland

2-3 business days

N/A

NSW

4-10 business days

2-3 business days

SA/Tas/Vic/WA

6-14 business days

2-3 business days

Overseas

N/A

5-14 business days

*Note: Delivery times are as provided by our carriers and are estimates only. Delays may occur during peak periods, and due to factors outside the carriers' control.

Standard shipping is $15 in Australia (free on orders over $200). Add $5 to ship Express (under 2kg only)

Pickup

Order online and collect from our warehouse in Townsville.

Location

Freight Charge

Ready for collection

11 Carlton Street, Kirwan

$0

Once invoice has been emailed
(Less than 1 business day)



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